Bacillus atrophaeus NX-12 Utilizes Exosmotic Glycerol from Fusarium oxysporum f. sp. cucumerinum for Fengycin Production

Bacillus strains are widely used as biological control agents to protect plants from fungal pathogens. However, whether Bacillus can exploit fungal pathogens to increase its biocontrol efficacy remains largely unexplored. Here, Bacillus atrophaeus NX-12 showed a high inhibition efficacy against Fusarium oxysporum f. sp. cucumerinum (FOC). The primary extracellular antifungal component of B. atrophaeus NX-12 was identified as fengycin by matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis. NX-12-secreted fengycin not only inhibited the germination of FOC spores but also induced the production of reactive oxygen species (ROS) in FOC cells, leading to oxidative stress and the accumulation of glycerol. Additionally, NX-12-secreted fengycin increased FOC cell wall hydrolase activity, leading to cell splitting and the exosmose of accumulated glycerol. The increased exosmose of glycerol further promoted the production of fengycin. Our results showed that in addition to the direct inhibition of FOC, NX-12 can indirectly strengthen its antagonistic efficacy against the pathogen by exploiting the exosmotic glycerol from FOC.