化学
干细胞
自体荧光
脂肪组织
发光
体内
荧光
生物物理学
归巢(生物学)
生物医学工程
细胞生物学
光电子学
光学
生物化学
材料科学
物理
生物
生物技术
医学
地球物理学
作者
Shuqi Wu,Chongwei Chi,Cheng‐Xiong Yang,Xiu‐Ping Yan
标识
DOI:10.1021/acs.analchem.6b00449
摘要
Reliable long-term in vivo tracking of stem cells is of great importance in stem cell-based therapy and research. Fluorescence imaging with in situ excitation has significant autofluorescence background, which results in poor signal-to-noise ratio (SNR). Here we report TAT penetrating peptide-bioconjugated long persistent luminescence nanoparticles (LPLNP-TAT) for long-term tracking of adipose-derived stem cells (ASC) without constant external excitation. LPLNP-TAT exhibits near-infrared emitting, red light renewable capability, and superior in vivo imaging depth and SNR compared with conventional organic dye and quantum dots. Our findings show that LPLNP-TAT can successfully label ASC without impairing their proliferation and differentiation and can effectively track ASC in skin-regeneration and tumor-homing models. We believe that LPLNP-TAT represents a new generation of cell tracking probes and will have broad application in diagnosis and therapy.
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