Determination of Binding Constants

This chapter contains sections titled: Theoretical Principles The Binding Constants and Binding Energies A General View on the Determination of Binding Constants Guideline for Experiments A Practical Course of Binding Constant Determination by UV/vis Spectroscopy Determination of Stoichiometry Evaluation of Complex Concentration Precautions to be Taken when Setting Up Concentration Conditions of the Titration Experiment Correlation between [H]0, [G]0, x and K How to Set Up [H]0 How to Set Up [G]0 Data Treatment General View Rose–Drago Method for UV/vis Spectroscopy Estimation of Error Conclusion for UV/vis Spectroscopic Method Practical Course of Action for NMR Spectroscopic Binding Constant Determination Determination of Stoichiometry Evaluation of Complex Concentration Data Treatment for NMR Method Rose–Drago Method for NMR Spectroscopy Estimation of Error for NMR Method Nonlinear Least Square Data Treatment of NMR Titration Method Estimation of Error for Nonlinear Least Square Method of NMR Spectroscopy Conclusion