绝对图塔
有害生物分析
寄主(生物学)
生物
选择(遗传算法)
突变体
夜行的
植物
生态学
基因
遗传学
计算机科学
人工智能
剑鱼科
作者
Yanhong Tang,Si-Yan Bi,Xiaodi Wang,Shun‐Xia Ji,Cong Huang,Guifen Zhang,Jiao Guo,Yang Ni,Dongfang Ma,Fang‐Hao Wan,Zengkui Lu,Wanxue Liu
标识
DOI:10.1016/j.ijbiomac.2024.130636
摘要
In insects, vision is crucial in finding host plants, but its role in nocturnal insects is largely unknown. Vision involves responses to specific spectra of photon wavelengths and opsins plays an important role in this process. Long-wavelength sensitive opsin (LW opsin) and blue-sensitive opsin (BL opsin) are main visual opsin proteins and play important in behavior regulation.We used CRISPR/Cas9 technology to mutate the long-wavelength-sensitive and blue wavelength-sensitive genes and explored the role of vision in the nocturnal invasive pest Tuta absoluta. Light wave experiments revealed that LW2 (−/−) and BL(−/−) mutants showed abnormal wavelength tropism. Both LW2 and BL mutations affected the preference of T. absoluta for the green environment. Mutations in LW2 and BL are necessary to inhibit visual attraction. The elimination of LW2 and BL affected the preference of leaf moths for green plants, and mutations in both induced a preference in moths for white plants. Behavioral changes resulting from LW2(−/−) and BL (−/−) mutants were not affected by sense of smell, further supporting the regulatory role of vision in insect behavior. To the best of our knowledge, this is the first study to reveal that vision, not smell, plays an important role in the host-seeking behavior of nocturnal insects at night, of which LW2 and BL opsins are key regulatory factors. These study findings will drive the development of the "vision-ecology" theory.
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